2016, ISBN: 3319327224

[EAN: 9783319327228], Neubuch, [PU: Springer International Publishing Mai 2016], BIOCHEMIE; CHEMIE / BIOCHEMIE (HEILMETHODE); ENZYM; MASSENSPEKTROMETRIE - SPEKTROMETRIE; POLYKETIDESYNTHAS… Meer...

[EAN: 9783319327228], Neubuch, [PU: Springer International Publishing Mai 2016], BIOCHEMIE; CHEMIE / BIOCHEMIE (HEILMETHODE); ENZYM; MASSENSPEKTROMETRIE - SPEKTROMETRIE; POLYKETIDESYNTHASES; MASSSPECTROMETRY; BIOSYNTHESIS,ENZYMES; KETOSYNTHASE; ACYLHYDROLASE; ENZYMATICDOMAINS; BIOCHEMICALENGINEERING, This item is printed on demand - it takes 3-4 days longer - Neuware -This thesis reports studies on the substrate specificity of crucial ketosynthase (KS) domains from trans-AT Polyketide Synthases (PKSs). Using a combination of electrospray ionisation-mass spectrometry (ESI-MS) and simple N-acetyl cysteamine (SNAC) substrate mimics, the specificity of a range of KS domains from the bacillaene and psymberin PKSs have been succsessfully studied with regard to the initial acylation step of KS-catalysis.In addition, the ability to alter the substrate tolerance of KS domains by simple point mutations in the active site has been demonstrated. A series of acyl-ACPs have been synthesised using a novel methodology and employed to probe the substrate specificity of both KS domains and the previously uncharcterised acyl hydrolase domain, PedC.KS-catalysed chain elongation reactions have also been conducted and monitored by ESI-MS/MS. All KS domains studied exhibited higher substrate specificity at the elongation step than in the preceeding acylation step. Furthermore, a mechanism of reversible acylation is proposed using the PsyA ACP1-KS1 di-domain. The findings in this thesis provide important insights into mechanisms of KS specificity and show that mutagenesis can be used to expand the repertoire of acceptable substrates for future PKS engineering. 196 pp. Englisch, Books<

2016, ISBN: 9783319327228

[ED: Hardcover], [PU: Springer / Springer International Publishing / Springer, Berlin], This thesis reports studies on the substrate specificity of crucial ketosynthase (KS) domains from … Meer...

[ED: Hardcover], [PU: Springer / Springer International Publishing / Springer, Berlin], This thesis reports studies on the substrate specificity of crucial ketosynthase (KS) domains from trans-AT Polyketide Synthases (PKSs). Using a combination of electrospray ionisation-mass spectrometry (ESI-MS) and simple N-acetyl cysteamine (SNAC) substrate mimics, the specificity of a range of KS domains from the bacillaene and psymberin PKSs have been succsessfully studied with regard to the initial acylation step of KS-catalysis. In addition, the ability to alter the substrate tolerance of KS domains by simple point mutations in the active site has been demonstrated. A series of acyl-ACPs have been synthesised using a novel methodology and employed to probe the substrate specificity of both KS domains and the previously uncharcterised acyl hydrolase domain, PedC. KS-catalysed chain elongation reactions have also been conducted and monitored by ESI-MS/MS. All KS domains studied exhibited higher substrate specificity at the elongation step than in the preceeding acylation step. Furthermore, a mechanism of reversible acylation is proposed using the PsyA ACP1-KS1 di-domain. The findings in this thesis provide important insights into mechanisms of KS specificity and show that mutagenesis can be used to expand the repertoire of acceptable substrates for future PKS engineering. 1st ed. 2016. 2016. xviii, 176 S. XVIII, 176 p. 136 illus., 99 illus. in color. 235 mm Versandfertig in 6-10 Tagen, DE, [SC: 0.00], Neuware, gewerbliches Angebot, Offene Rechnung (Vorkasse vorbehalten)<

2016, ISBN: 9783319327228

[ED: Gebunden], [PU: Springer International Publishing], This thesis reports studies on the substrate specificity of crucial ketosynthase (KS) domains from trans-AT Polyketide Synthases (… Meer...

[ED: Gebunden], [PU: Springer International Publishing], This thesis reports studies on the substrate specificity of crucial ketosynthase (KS) domains from trans-AT Polyketide Synthases (PKSs). Using a combination of electrospray ionisation-mass spectrometry (ESI-MS) and simple N-acetyl cysteamine (SNAC) substrate mimics, the specificity of a range of KS domains from the bacillaene and psymberin PKSs have been succsessfully studied with regard to the initial acylation step of KS-catalysis. In addition, the ability to alter the substrate tolerance of KS domains by simple point mutations in the active site has been demonstrated. A series of acyl-ACPs have been synthesised using a novel methodology and employed to probe the substrate specificity of both KS domains and the previously uncharcterised acyl hydrolase domain, PedC. KS-catalysed chain elongation reactions have also been conducted and monitored by ESI-MS/MS. All KS domains studied exhibited higher substrate specificity at the elongation step than in the preceeding acylation step...., DE, [SC: 0.00], Neuware, gewerbliches Angebot, 196, [GW: 465g], 1st ed. 2016, Banküberweisung, PayPal, Klarna-Sofortüberweisung, [CT: Sonstiges / Sonstiges]<

2016, ISBN: 3319327224

1st ed. 2016 Gebundene Ausgabe Biochemie, Chemie / Biochemie, Biochemie (Heilmethode), Enzym, Massenspektrometrie - Spektrometrie , Medizinische Chemie, Pharmazeutische Chemie, Chemisc… Meer...

1st ed. 2016 Gebundene Ausgabe Biochemie, Chemie / Biochemie, Biochemie (Heilmethode), Enzym, Massenspektrometrie - Spektrometrie , Medizinische Chemie, Pharmazeutische Chemie, Chemische Biologie, Biotechnologie, PolyketideSynthases; massspectrometry; Biosynthesis,Enzymes; Ketosynthase; AcylHydrolase; EnzymaticDomains; biochemicalengineering, mit Schutzumschlag 11, [PU:Springer International Publishing; Springer International Publishing AG]<