2009, ISBN: 9783639173062

A recombinant S100A4 protein was produced in Pichia pastoris (Pichia). The binding capacity to the hydrophobic material was rather low, but the eluted protein did however show an acceptab… Meer...

A recombinant S100A4 protein was produced in Pichia pastoris (Pichia). The binding capacity to the hydrophobic material was rather low, but the eluted protein did however show an acceptable purity for utilization in downstream functional assays. .contentData { line-height: 14px; margin: 2px; padding: 2px; } .contentData table.mainTable { font-family:Arial, sans-serif, Verdana; font-size:13px; } .contentData td { padding-right:10px; line-height: 16px; } .contentData td.header { width: 150px; font-weight: bold; } .contentData ul { padding-left: 40px; } .contentData h2 { font-family:Arial, sans-serif, Verdana; font-size: 24px; margin: 18px 0 18px 0; font-weight: 700; } .contentData h3 { font-family:Arial, sans-serif, Verdana; font-size: 18px; margin: 18px 0 18px 0; font-weight: 700; } .contentData p { font-family:Arial, sans-serif, Verdana; font-size: 12px; margin: 12px 0 12px 0; } .contentData li { font-family:Arial, sans-serif, Verdana!important; font-size: 12px; } Allgemein Titel: Expression, purification and cellular uptake of a S100A4 protein - an agent promoting metastasis Genre: Biochemie Medientyp: Taschenbuch Beschreibung A recombinant S100A4 protein was produced in Pichia <br />pastoris (Pichia). The expression vector contained <br />parts of the AOX1 promoter and also a secretion <br />signal, making it possible to purify the protein <br />from the yeast media after methanol<br />induction. Attempts were made to purify the protein <br />from the media both by hydrophobic interaction<br />chromatography (HIC) and by anionic ion exchange <br />chromatography (IEC). The binding capacity to<br />the hydrophobic material was rather low, but the <br />eluted protein did however show an acceptable purity<br />for utilization in downstream functional assays. The <br />protein eluted from the hydrophobic column was <br />labelled with the fluorescent dye (Cy3) and used for <br />internalization studies in the human cell line HeLa. <br />Similar to a mouse rS100A4 produced in bacterial <br />cells, the Pichia produced protein was also found <br />internalized into the HeLa cells. The biological <br />significance of these findings is at present not <br />elucidated and will be further<br />studied. Weitere Informationen Sprache: Englisch Seitenanzahl: 152 Verlag: VDM Verlag Dr. Müller Mitwirkende Autor: Sjøli, Stian , Neu, Festpreisangebot, [LT: FixedPrice], EAN: 9783639173062, Format: Taschenbuch, Anzahl der Seiten: 152 Seiten, Publikationsname: Expression, Purification And Cellular Uptake of A S100a4 Protein, Sprache: Englisch, Vdm Verlag, 2009<

ISBN: 9783639173062

A recombinant S100A4 protein was produced in Pichia pastoris (Pichia). The expression vector contained parts of the AOX1 promoter and also a secretion signal, making it possible to purify… Meer...

A recombinant S100A4 protein was produced in Pichia pastoris (Pichia). The expression vector contained parts of the AOX1 promoter and also a secretion signal, making it possible to purify the protein from the yeast media after methanolinduction. Attempts were made to purify the protein from the media both by hydrophobic interactionchromatography (HIC) and by anionic ion exchange chromatography (IEC). The binding capacity tothe hydrophobic material was rather low, but the eluted protein did however show an acceptable purityfor utilization in downstream functional assays. The protein eluted from the hydrophobic column was labelled with the fluorescent dye (Cy3) and used for internalization studies in the human cell line HeLa. Similar to a mouse rS100A4 produced in bacterial cells, the Pichia produced protein was also found internalized into the HeLa cells. The biological significance of these findings is at present not elucidated and will be furtherstudied. Bücher > Fremdsprachige Bücher > Englische Bücher 220 x 150 x 9 mm , VDM, Taschenbuch, VDM<

ISBN: 9783639173062

*Expression purification and cellular uptake of a S100A4 protein* - an agent promoting metastasis / Taschenbuch für 58.99 € / Aus dem Bereich: Bücher, Wissenschaft, Biologie Medien > Büch… Meer...

*Expression purification and cellular uptake of a S100A4 protein* - an agent promoting metastasis / Taschenbuch für 58.99 € / Aus dem Bereich: Bücher, Wissenschaft, Biologie Medien > Bücher nein Buch (kartoniert) Hardcover;Naturwissenschaften, Medizin, Informatik, Technik;Biochemie, Biophysik, VDM Verlag Dr. Müller<

2017, ISBN: 3639173066

[EAN: 9783639173062], Neubuch, [PU: VDM Verlag], EXPRESSION, PURIFICATION AND CELLULAR UPTAKE OF A S100A4 PROTEIN, PRINT ON DEMAND Book; New; Publication Year 2017; Fast Shipping from the… Meer...

[EAN: 9783639173062], Neubuch, [PU: VDM Verlag], EXPRESSION, PURIFICATION AND CELLULAR UPTAKE OF A S100A4 PROTEIN, PRINT ON DEMAND Book; New; Publication Year 2017; Fast Shipping from the UK., Books<

2009, ISBN: 3639173066

[EAN: 9783639173062], Neubuch, [PU: VDM Verlag 2009-07], Books