ISBN: 9783843391764

Among 128 proteolytic bacterial isolates, Brevibacterium otitidis, CBS-76 was choosen on the basis of secreted protease. Analysis of purified protease revealed an apparent molecular weigh… Meer...

Among 128 proteolytic bacterial isolates, Brevibacterium otitidis, CBS-76 was choosen on the basis of secreted protease. Analysis of purified protease revealed an apparent molecular weight of 47 KDa with 15.2% glutamic acid content. The purified protease showed the maximum activity after 68h at 35ºC and pH 7.2. The purified enzyme was inhibited in the presence of EDTA but maximally activated in the presence of sodium arsenate. LD50 on mice was 8 µg /100 g body weight. Histological study using transmission electron microscope revealed that nuclei of treated mice hepatocytes appeared with inactive heterochromatin and the nucleoli tend to disintegrate. While the cytoplasm contains swollen mitochondria and some destructed mitochondria lost their inner cristae. And fat droplets accumulated in the cytoplasm in the form of empty vacuoles(steatosis). On studying, novel inhibitors derived from common drugs derivatives of saccharin sodium,compound 6 and 9 showed the maximum percentage of enzyme inhibition. Bacterial proteases production, purification and partial characterization Buch (fremdspr.) Bücher>Fremdsprachige Bücher>Englische Bücher, LAP LAMBERT Academic Publishing<

2011, ISBN: 9783843391764

Among 128 proteolytic bacterial isolates, Brevibacterium otitidis, CBS-76 was choosen on the basis of secreted protease. Analysis of purified protease revealed an apparent molecular weigh… Meer...

Among 128 proteolytic bacterial isolates, Brevibacterium otitidis, CBS-76 was choosen on the basis of secreted protease. Analysis of purified protease revealed an apparent molecular weight of 47 KDa with 15.2% glutamic acid content. The purified protease showed the maximum activity after 68h at 35ºC and pH 7.2. The purified enzyme was inhibited in the presence of EDTA but maximally activated in the presence of sodium arsenate. LD50 on mice was 8 µg /100 g body weight. Histological study using transmission electron microscope revealed that nuclei of treated mice hepatocytes appeared with inactive heterochromatin and the nucleoli tend to disintegrate. While the cytoplasm contains swollen mitochondria and some destructed mitochondria lost their inner cristae. And fat droplets accumulated in the cytoplasm in the form of empty vacuoles(steatosis). On studying, novel inhibitors derived from common drugs derivatives of saccharin sodium,compound 6 and 9 showed the maximum percentage of enzyme inhibition. Buch (fremdspr.) Essam Kotb Taschenbuch, LAP LAMBERT Academic Publishing, 17.01.2011, LAP LAMBERT Academic Publishing, 2011<

ISBN: 9783843391764

Among 128 proteolytic bacterial isolates, Brevibacterium otitidis, CBS-76 was choosen on the basis of secreted protease. Analysis of purified protease revealed an apparent molecular weigh… Meer...

Among 128 proteolytic bacterial isolates, Brevibacterium otitidis, CBS-76 was choosen on the basis of secreted protease. Analysis of purified protease revealed an apparent molecular weight of 47 KDa with 15.2% glutamic acid content. The purified protease showed the maximum activity after 68h at 35ºC and pH 7.2. The purified enzyme was inhibited in the presence of EDTA but maximally activated in the presence of sodium arsenate. LD50 on mice was 8 mig /100 g body weight. Histological study using transmission electron microscope revealed that nuclei of treated mice hepatocytes appeared with inactive heterochromatin and the nucleoli tend to disintegrate. While the cytoplasm contains swollen mitochondria and some destructed mitochondria lost their inner cristae. And fat droplets accumulated in the cytoplasm in the form of empty vacuoles(steatosis). On studying, novel inhibitors derived from common drugs derivatives of saccharin sodium,compound 6 and 9 showed the maximum percentage of enzyme inhibition. Bücher > Fremdsprachige Bücher > Englische Bücher 220 x 150 x 11 mm , LAP LAMBERT Academic Publishing, LAP LAMBERT Academic Publishing<

ISBN: 9783843391764

Paperback, [PU: LAP Lambert Academic Publishing], Among 128 proteolytic bacterial isolates, Brevibacterium otitidis, CBS-76 was choosen on the basis of secreted protease. Analysis of puri… Meer...

Paperback, [PU: LAP Lambert Academic Publishing], Among 128 proteolytic bacterial isolates, Brevibacterium otitidis, CBS-76 was choosen on the basis of secreted protease. Analysis of purified protease revealed an apparent molecular weight of 47 KDa with 15.2% glutamic acid content. The purified protease showed the maximum activity after 68h at 35C and pH 7.2. The purified enzyme was inhibited in the presence of EDTA but maximally activated in the presence of sodium arsenate. LD50 on mice was 8 μg /100 g body weight. Histological study using transmission electron microscope revealed that nuclei of treated mice hepatocytes appeared with inactive heterochromatin and the nucleoli tend to disintegrate. While the cytoplasm contains swollen mitochondria and some destructed mitochondria lost their inner cristae. And fat droplets accumulated in the cytoplasm in the form of empty vacuoles(steatosis). On studying, novel inhibitors derived from common drugs derivatives of saccharin sodium, compound 6 and 9 showed the maximum percentage of enzyme inhibition., Microbiology (non-medical)<

ISBN: 3843391769

Taschenbuch, [EAN: 9783843391764], LAP LAMBERT Academic Publishing, LAP LAMBERT Academic Publishing, Book, [PU: LAP LAMBERT Academic Publishing], LAP LAMBERT Academic Publishing, 56491011… Meer...

Taschenbuch, [EAN: 9783843391764], LAP LAMBERT Academic Publishing, LAP LAMBERT Academic Publishing, Book, [PU: LAP LAMBERT Academic Publishing], LAP LAMBERT Academic Publishing, 56491011, Mikrobiologie, 56458011, Biowissenschaften, 56047011, Wissenschaft, 54071011, Genres, 52044011, Fremdsprachige Bücher<