Expanded Bed Chromatography - pocketboek

EAN (ISBN-13): 9789048153800
ISBN (ISBN-10): 9048153808
Gebonden uitgave
pocket book
Verschijningsjaar: 2010
Uitgever: Mattiasson, Bo, Springer
280 Bladzijden
Gewicht: 0,530 kg
Taal: eng/Englisch

Boek bevindt zich in het datenbestand sinds 2011-10-03T15:07:57+02:00 (Amsterdam)
Detailpagina laatst gewijzigd op 2023-06-08T13:38:44+02:00 (Amsterdam)
ISBN/EAN: 9789048153800

ISBN - alternatieve schrijfwijzen:
90-481-5380-8, 978-90-481-5380-0
alternatieve schrijfwijzen en verwante zoekwoorden:
Titel van het boek: chromatography, going bed, bed with

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Gegevens van de uitgever

Auteur: B. Mattiasson
Titel: Expanded Bed Chromatography
Uitgeverij: Springer; Springer Netherland
272 Bladzijden
Verschijningsjaar: 2010-12-15
Dordrecht; NL
Gedrukt / Gemaakt in
Taal: Engels
106,99 € (DE)
109,99 € (AT)
118,00 CHF (CH)
POD
VI, 272 p.

BC; Hardcover, Softcover / Technik/Chemische Technik; Biotechnologie; Verstehen; Chromatography; DNA; Downstream Processing; Fermentation; Glucose; Scale up; gene therapy; proteins; biochemical engineering; Chemical Bioengineering; Physical Chemistry; Biotechnology; Physikalische Chemie; BB

Expanded bed adsorption: elution in expanded bed mode.- Monitoring, modeling, and control strategies for expanded-bed adsorption processes.- Factors affecting dispersion in expanded bed chromatography.- Physical and biochemical characterization of a simple intermediate between fluidized and expanded bed contactors.- On-line monitoring of the purification of GST-(His)6 from an unclarified Escherichia coli homogenate within an immobilised metal affinity expanded bed.- The effect of column verticality on separation efficiency in expanded bed adsorption.- Evaluation of the effect of in-bed sampling on expanded bed adsorption.- EBA columns with a distribution system based on local stirring.- Simplified and more robust EBA processes by elution in expanded bed mode.- Cell/adsorbent interactions in expanded bed adsorption of proteins.- Validation issues related to expanded bed technology.- Highly dense beaded sorbents suitable for fluidized bed applications.- Characterization of very dense mineral oxide—gel composites for fluidized-bed adsorption of biomolecules.- Expanded bed adsorption on supermacroporous cross-linked cellulose matrix.- Characterisation of STREAMLINETM Phenyl.- Use of Streamline chelating for capture and purification of poly-His-tagged recombinant proteins.- A simplified purification procedure of a-lactalbumin from milk using Ca2+-dependent adsorption in hydrophobic expanded bed chromatography.- Expanded bed adsorption as a unique unit operation for the isolation of bacteriocins from fermentation media.- Pilot scale recovery of monoclonal antibodies by expanded bed ion exchange adsorption.- Capture of human Fab fragments by expanded bed adsorption with a mixed mode adsorbent.- Purification of Recombinant Human Serum Albumin. Efficient purification usingSTREAMLINE.- Direct coupling of expanded bed adsorption with a downstream purification step.- Production of plasmid DNA for human gene therapy using modified alkaline cell lysis and expanded bed anion exchange chromatography.- Development and scale up of a capture step (expanded bed chromatography) for a fusion protein expressed intracellularly in Escherichia coli.- On-line monitoring of glucose and/or lactate in a fermentation process using an expanded micro-bed flow injection analyzer.- Binding assays in heterogeneous media using a flow injection system with an expanded micro-bed adsorption column.- Use of a micro-expanded bed containing immobilised lysozyme for cell disruption in flow injection analysis.- Flow injection analysis of intracellular ?-galactosidase in Escherichia coli cultivations, using an on-line system including cell disruption, debris separation and immunochemical quantification.- Key word Index.- Author Index.