2015, ISBN: 9789048154029

[ED: Taschenbuch], [PU: Springer Netherlands], Neuware - It is thirty years since the technique of high-yield preparation of isolated hepatocytes, by collagenase perfusion of the liver, w… Meer...

[ED: Taschenbuch], [PU: Springer Netherlands], Neuware - It is thirty years since the technique of high-yield preparation of isolated hepatocytes, by collagenase perfusion of the liver, was published. The original method described by Berry and Friend has undergone many minor modifications by other workers, and the two-step procedure introduced by Seglen in 1976 has become the most frequent way to prepare hepatocyte suspensions. An important development introduced by Bissell in 1973 was the use of the cells as the first step in monolayer culture. The availability of the isolated hepatocyte preparation as cells in suspension or culture has undoubtedly facilitated research on the liver. This was emphasised in our book, published (with Dr. Greg Barritt) in 1990, which described in detail methods of preparation and the properties of the isolated hepatocytes. It also discussed the usefulness of the preparation for the study of intermediary and xenobiotic metabolism, calcium ion transport, and the growth and differentiation of hepatocytes in culture. The book also touched briefly on a range of specialised techniques, including peri fusion, subcellular fractionation, transplantation, cryopreservation and measurement of intracellular pH. Although standard procedures for the manipulation of hepatocytes have not changed a great deal in ten years, they have undoubtedly been refined. This applies particularly to hepatocyte culture techniques, cryopreservation, and even to preparation of hepatocyte suspensions, where it is now feasible to use purified enzymes. There is also much more emphasis on the use and study of human hepatocytes, particularly in the field of pharmacology and therapeutics.- Besorgungstitel - vorauss. Lieferzeit 3-5 Tage., DE, [SC: 0.00], Neuware, gewerbliches Angebot, 235x155x34 mm, 624, [GW: 931g], Banküberweisung, Offene Rechnung, Kreditkarte, PayPal, Offene Rechnung (Vorkasse vorbehalten), International shipping<

2010, ISBN: 9048154022

[EAN: 9789048154029], New book, [SC: 12.24], [PU: Springer Netherlands], ANIMALS; APOPTOSIS; BIOLOGY; CELL; CELLCYCLE; CYTOCHROMEP450; DEVELOPMENT; GENE; GENETHERAPY; MEMBRANE; METABOLISM… Meer...

[EAN: 9789048154029], New book, [SC: 12.24], [PU: Springer Netherlands], ANIMALS; APOPTOSIS; BIOLOGY; CELL; CELLCYCLE; CYTOCHROMEP450; DEVELOPMENT; GENE; GENETHERAPY; MEMBRANE; METABOLISM; MITOCHONDRIA; REGULATION; SYSTEM, Druck auf Anfrage Neuware - Printed after ordering - It is thirty years since the technique of high-yield preparation of isolated hepatocytes, by collagenase perfusion of the liver, was published. The original method described by Berry and Friend has undergone many minor modifications by other workers, and the two-step procedure introduced by Seglen in 1976 has become the most frequent way to prepare hepatocyte suspensions. An important development introduced by Bissell in 1973 was the use of the cells as the first step in monolayer culture. The availability of the isolated hepatocyte preparation as cells in suspension or culture has undoubtedly facilitated research on the liver. This was emphasised in our book, published (with Dr. Greg Barritt) in 1990, which described in detail methods of preparation and the properties of the isolated hepatocytes. It also discussed the usefulness of the preparation for the study of intermediary and xenobiotic metabolism, calcium ion transport, and the growth and differentiation of hepatocytes in culture. The book also touched briefly on a range of specialised techniques, including peri fusion, subcellular fractionation, transplantation, cryopreservation and measurement of intracellular pH. Although standard procedures for the manipulation of hepatocytes have not changed a great deal in ten years, they have undoubtedly been refined. This applies particularly to hepatocyte culture techniques, cryopreservation, and even to preparation of hepatocyte suspensions, where it is now feasible to use purified enzymes. There is also much more emphasis on the use and study of human hepatocytes, particularly in the field of pharmacology and therapeutics., Books<

ISBN: 9789048154029

It is thirty years since the technique of high-yield preparation of isolated hepaytes, by collagenase perfusion of the liver, was published. The original method described by Berry and Fri… Meer...

It is thirty years since the technique of high-yield preparation of isolated hepaytes, by collagenase perfusion of the liver, was published. The original method described by Berry and Friend has undergone many minor modifications by other workers, and the two-step procedure introduced by Seglen in 1976 has become the most frequent way to prepare hepayte suspensions. An important development introduced by Bissell in 1973 was the use of the cells as the first step in monolayer culture. The availability of the isolated hepayte preparation as cells in suspension or culture has undoubtedly facilitated research on the liver. This was emphasised in our book, published (with Dr. Greg Barritt) in 1990, which described in detail methods of preparation and the properties of the isolated hepaytes. It also discussed the usefulness of the preparation for the study of intermediary and xenobiotic metabolism, calcium ion transport, and the growth and differentiation of hepaytes in culture. The book also touched briefly on a range of specialised techniques, including peri fusion, subcellular fractionation, transplantation, cryopreservation and measurement of intracellular pH. Although standard procedures for the manipulation of hepaytes have not changed a great deal in ten years, they have undoubtedly been refined. This applies particularly to hepayte culture techniques, cryopreservation, and even to preparation of hepayte suspensions, where it is now feasible to use purified enzymes. There is also much more emphasis on the use and study of human hepaytes, particularly in the field of pharmacology and therapeutics. New Textbooks>Trade Paperback>Nature & Wildlife>Nature & Wildlife Writing>Nature & Wildlife Writing, Springer Netherlands Core >1 >T<

2010, ISBN: 9048154022

2000 Kartoniert / Broschiert Klinische und Innere Medizin, Tiermedizin, animals; apoptosis; Biology; Cell; cellcycle; CytochromeP450; Development; Gene; genetherapy; membrane; metabolis… Meer...

2000 Kartoniert / Broschiert Klinische und Innere Medizin, Tiermedizin, animals; apoptosis; Biology; Cell; cellcycle; CytochromeP450; Development; Gene; genetherapy; membrane; metabolism; mitochondria; Regulation; system, with dust cover 11, [PU:Springer Netherlands; Springer Netherland]<