ISBN: 9789048162666

This text is aimed at a student readership but will also be useful to life science researchers faced with the task of isolating a protein.The logic of the overall approach to protein isol… Meer...

This text is aimed at a student readership but will also be useful to life science researchers faced with the task of isolating a protein.The logic of the overall approach to protein isolation is explained and the physical principles of each separation method are made clear by the use of simple models and analogies drawn from everyday experiences. The author's aim has been to deepen the readers' insight into protein isolation methods, so that they may tackle new problems and perhaps devise new approaches to old problems. Many of the methods described are drawn from the author's own research and are thus uniquely described here; examples are three-phase partitioning, non-linear electrophoresis and a simple approach to buffer making. In this 2nd edition, the treatment of the basic physical principles has been expanded and clarified, the importance of ionic strength in measuring enzyme pH optima is emphasised and a computer program for the calculation of buffers of defined ionic strength is provided. The section on three-phase partitioning has been expanded to include the latest research findings on the use of t-butanol to inhibit enzymes and minimise homogenisation artefacts, the treatment of HPLC has been expanded and the most common practical methods are explained in detail in a new chapter. Additional study questions are provided, as are the answers to all study questions. Trade Books>Trade Paperback>Science>Biology>Biology, Springer Netherlands Core >1<

ISBN: 9789048162666

It is a truism of science that the more fundamental the subject, the more universally applicable it is. Neverthelens, it is important to strike a level of fundamentalness appropriate to t… Meer...

It is a truism of science that the more fundamental the subject, the more universally applicable it is. Neverthelens, it is important to strike a level of fundamentalness appropriate to the task in hand. For example, an in-depth study of the mechanics of motor cars would tell one nothing about the dynamics of traffic. Traffic exists on a different level - it is dependent upon the existence of motor vehicles but the physics and mathematics of traffic can be adequately addressed by considering motor vehicles as mobile blobs, with no consideration of how they become mobile. To start a discourse on traffic with a consideration of the mechanics of motor vehicles would thus be inappropropriate. In writing this volume, I have wrestled with the question of the appropriate level at which to address the physics underlying many of the techniques used in protein isolation. I have tried to strike a level as would be used by a mechanic (with perhaps a slight leaning towards an engineer) - i.e. a practical level, offering appropriate insight but with minimal mathematics. Some people involved in biochemical research have a minimal grounding in chemistry and physics and so I have tried to keep it as simple as possible. Trade Books>Trade Paperback>Science>Biology>Biology, Springer Netherlands Core >1<

2010, ISBN: 9789048162666

[ED: Kartoniert / Broschiert], [PU: Springer Netherlands], Dieser Artikel ist ein Print on Demand Artikel und wird nach Ihrer Bestellung fuer Sie gedruckt. It is a truism of science that … Meer...

[ED: Kartoniert / Broschiert], [PU: Springer Netherlands], Dieser Artikel ist ein Print on Demand Artikel und wird nach Ihrer Bestellung fuer Sie gedruckt. It is a truism of science that the more fundamental the subject, the more universally applicable it is. Neverthelens, it is important to strike a level of fund, DE, [SC: 0.00], Neuware, gewerbliches Angebot, Taschenbuch, 249, [GW: 381g], 2nd edition, Banküberweisung, PayPal, [CT: Naturwissenschaft/Mathematik / Sonstiges - Naturwissenschaften]<

2010, ISBN: 9789048162666

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Erscheinungsdatum: 15.12.2010, Medium: Taschenbuch, Einband: Kartoniert / Broschiert, Titel: A Guide to Protein Isolation, Auflage: 2. Auflage von 2010 // Softcover reprint of hardcover 2… Meer...

Erscheinungsdatum: 15.12.2010, Medium: Taschenbuch, Einband: Kartoniert / Broschiert, Titel: A Guide to Protein Isolation, Auflage: 2. Auflage von 2010 // Softcover reprint of hardcover 2nd ed. 2003, Autor: Dennison, C., Verlag: Springer Netherlands // Springer Netherland, Sprache: Englisch, Schlagworte: Chemie, Rubrik: Biochemie // Biophysik, Seiten: 268, Informationen: Paperback, Gewicht: 468 gr, Verkäufer: averdo Sachbücher<

2003, ISBN: 9789048162666

*A Guide to Protein Isolation A Guide to Protein Isolation * - Softcover reprint of hardcover 2nd ed. 2003 / Taschenbücher für 106.99€ / Aus dem Bereich: Bücher-Wissenschaft-Biologie Tasc… Meer...

*A Guide to Protein Isolation A Guide to Protein Isolation * - Softcover reprint of hardcover 2nd ed. 2003 / Taschenbücher für 106.99€ / Aus dem Bereich: Bücher-Wissenschaft-Biologie Taschenbücher Buch (kartoniert) no Medien > Bücher Hardcover > Naturwissenschaften, Medizin, Informatik, Technik > Biochemie, Biophysik, Springer Netherlands<