ISBN: 9789048162666

This text is aimed at a student readership but will also be useful to life science researchers faced with the task of isolating a protein.The logic of the overall approach to protein isol… Meer...

This text is aimed at a student readership but will also be useful to life science researchers faced with the task of isolating a protein.The logic of the overall approach to protein isolation is explained and the physical principles of each separation method are made clear by the use of simple models and analogies drawn from everyday experiences. The author's aim has been to deepen the readers' insight into protein isolation methods, so that they may tackle new problems and perhaps devise new approaches to old problems. Many of the methods described are drawn from the author's own research and are thus uniquely described here; examples are three-phase partitioning, non-linear electrophoresis and a simple approach to buffer making. In this 2nd edition, the treatment of the basic physical principles has been expanded and clarified, the importance of ionic strength in measuring enzyme pH optima is emphasised and a computer program for the calculation of buffers of defined ionic strength is provided. The section on three-phase partitioning has been expanded to include the latest research findings on the use of t-butanol to inhibit enzymes and minimise homogenisation artefacts, the treatment of HPLC has been expanded and the most common practical methods are explained in detail in a new chapter. Additional study questions are provided, as are the answers to all study questions. Trade Books>Trade Paperback>Science>Biology>Biology, Springer Netherlands Core >1<

2010, ISBN: 9789048162666

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Erscheinungsdatum: 15.12.2010, Medium: Taschenbuch, Einband: Kartoniert / Broschiert, Titel: A Guide to Protein Isolation, Auflage: 2. Auflage von 2010 // Softcover reprint of hardcover 2… Meer...

Erscheinungsdatum: 15.12.2010, Medium: Taschenbuch, Einband: Kartoniert / Broschiert, Titel: A Guide to Protein Isolation, Auflage: 2. Auflage von 2010 // Softcover reprint of hardcover 2nd ed. 2003, Autor: Dennison, C., Verlag: Springer Netherlands // Springer Netherland, Sprache: Englisch, Schlagworte: Chemie, Rubrik: Biochemie // Biophysik, Seiten: 268, Informationen: Paperback, Gewicht: 468 gr, Verkäufer: averdo Sachbücher<

2010, ISBN: 9048162661

gebonden uitgave

Softcover reprint of hardcover 2nd ed. 2003 Kartoniert / Broschiert Chemie, Chromatography; Enzyme; enzymes; proteins, mit Schutzumschlag 11, [PU:Springer Netherlands; Springer Netherland]

2003, ISBN: 9048162661

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A Guide to Protein Isolation ab 119.99 € als Taschenbuch: Softcover reprint of hardcover 2nd ed. 2003. Aus dem Bereich: Bücher, Taschenbücher, Naturwissenschaft, Springer Netherlands

2003, ISBN: 9048162661

gebonden uitgave

A Guide to Protein Isolation ab 119.99 EURO Softcover reprint of hardcover 2nd ed. 2003 Medien > Bücher