2010, ISBN: 9789048169115

Kartoniert, 328 Seiten, 235mm x 155mm x 21mm, Sprache(n): eng Comprehensive review of all methods suitable for T cell responses Prepared by pioneers in each respective assay Inclusive … Meer...

Kartoniert, 328 Seiten, 235mm x 155mm x 21mm, Sprache(n): eng Comprehensive review of all methods suitable for T cell responses Prepared by pioneers in each respective assay Inclusive of strategies for global monitoring at the systemic and peripheral (target organ) levelActive specific immunotherapy is a promising but investigational modality in the management of cancer patients. Currently, several different cancer vaccine formulations such as peptides, proteins, antigen-pulsed dendritic cells, whole tumor cells, etc. in combination with various adjuvants and carriers are being evaluated in clinical trials (1-3). To determine the optimal cancer vaccine strategy, a surrogate immunological end-point that correlates with clinical outcome needs to be defined, since it would facilitate the rapid comparison of these various formulations. Traditional immunological assays such as ELISA, proliferation and cytotoxicity assays can detect immune responses in vaccinated patients but are not quantitative. In contrast, novel assays such as enzyme-linked immunospot (ELISPOT) assay, intracellular cytokine assay and tetramer assay can quantitate the frequency of antigen-specific T cells. Of these, the ELISPOT assay has the 5 lowest detection limit with 1/10 peripheral blood mononuclear cells (PBMC) and has been determined to be one of the most useful assays to evaluate immune response to cancer vaccines (4). However, the IFN-? ELISPOT assay is not an exclusive measure of cytotoxic T-lymphocyte (CTL) activity as non-cytotoxic cells can also secrete IFN-?. Additionally, CTL with lytic activity do not always secrete IFN-? (5). A more relevant approach to assess functional activity of cytotoxic lymphocytes would be to measure the secretion of molecules that are associated with lytic activity. One of the major mechanisms of cell-mediated cytotoxicity involves exocytosis of cytoplasmic granules from the effector toward the target cell. Versandkostenfreie Lieferung Antigen, Cancer, Cytokine, Cytotoxicity, Gene, gene expression, immunotherapy, Lymphocytes, immunology, Proliferation, vaccine, virus<

2010, ISBN: 9789048169115

[ED: Softcover], [PU: Springer Netherlands], Active specific immunotherapy is a promising but investigational modality in the management of cancer patients. Currently, several different c… Meer...

[ED: Softcover], [PU: Springer Netherlands], Active specific immunotherapy is a promising but investigational modality in the management of cancer patients. Currently, several different cancer vaccine formulations such as peptides, proteins, antigen-pulsed dendritic cells, whole tumor cells, etc. in combination with various adjuvants and carriers are being evaluated in clinical trials (1-3). To determine the optimal cancer vaccine strategy, a surrogate immunological end-point that correlates with clinical outcome needs to be defined, since it would facilitate the rapid comparison of these various formulations. Traditional immunological assays such as ELISA, proliferation and cytotoxicity assays can detect immune responses in vaccinated patients but are not quantitative. In contrast, novel assays such as enzyme-linked immunospot (ELISPOT) assay, intracellular cytokine assay and tetramer assay can quantitate the frequency of antigen-specific T cells. Of these, the ELISPOT assay has the 5 lowest detection limit with 1/10 peripheral blood mononuclear cells (PBMC) and has been determined to be one of the most useful assays to evaluate immune response to cancer vaccines (4). However, the IFN-? ELISPOT assay is not an exclusive measure of cytotoxic T-lymphocyte (CTL) activity as non-cytotoxic cells can also secrete IFN-?. Additionally, CTL with lytic activity do not always secrete IFN-? (5). A more relevant approach to assess functional activity of cytotoxic lymphocytes would be to measure the secretion of molecules that are associated with lytic activity. One of the major mechanisms of cell-mediated cytotoxicity involves exocytosis of cytoplasmic granules from the effector toward the target cell. 2010. xii, 314 S. 6 Farbabb. 240 mm Versandfertig in 6-10 Tagen, DE, [SC: 0.00], Neuware, gewerbliches Angebot, Offene Rechnung (Vorkasse vorbehalten)<

2010, ISBN: 9789048169115

Springer, Taschenbuch, Auflage: Softcover reprint of hardcover 1st ed. 2005, 328 Seiten, Publiziert: 2010-10-19T00:00:01Z, Produktgruppe: Book, Medizin, Kategorien, Bücher, Pathologie, Gr… Meer...

Springer, Taschenbuch, Auflage: Softcover reprint of hardcover 1st ed. 2005, 328 Seiten, Publiziert: 2010-10-19T00:00:01Z, Produktgruppe: Book, Medizin, Kategorien, Bücher, Pathologie, Grundlagen, Medizin & Gesundheitsdienste, Fachbücher, Immunologie, Hämatologie, Klinische Medizin, Onkologie, Erkrankungen, Nosologie, Verwaltung & Richtlinien, Ingenieurwissenschaft & Technik, Naturwissenschaften & Technik, Mikrobiologie, Biologie, Biowissenschaften, Nagorsen, Dirk, Marincola, Francesco M. Springer, 2010<

2010, ISBN: 9789048169115

Springer, Taschenbuch, Auflage: Softcover reprint of hardcover 1st ed. 2005, 328 Seiten, Publiziert: 2010-10-19T00:00:01Z, Produktgruppe: Book, Medizin, Kategorien, Bücher, Pathologie, Gr… Meer...

Springer, Taschenbuch, Auflage: Softcover reprint of hardcover 1st ed. 2005, 328 Seiten, Publiziert: 2010-10-19T00:00:01Z, Produktgruppe: Book, Medizin, Kategorien, Bücher, Pathologie, Grundlagen, Medizin & Gesundheitsdienste, Fachbücher, Immunologie, Hämatologie, Klinische Medizin, Onkologie, Erkrankungen, Nosologie, Verwaltung & Richtlinien, Ingenieurwissenschaft & Technik, Naturwissenschaften & Technik, Mikrobiologie, Biologie, Biowissenschaften, Nagorsen, Dirk, Marincola, Francesco M. Springer, 2010<

2010, ISBN: 9789048169115

How to analyze cellular immune responses against tumor associated antigens, Buch, Softcover, Softcover reprint of hardcover 1st ed. 2005, [PU: Springer], Springer, 2010