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ISBN: 9789401014144

Up to about twenty-five years ago, virtually the entire field of microscopy could be overseen and even practized by any active research worker. The rapid evolution which microscopy in its… Meer...

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Light microscopic techniques in biology and medicine - nieuw boek

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Medicine & Public Health; Anatomy; Veterinary Medicine/Veterinary Science Laboratory, biology, care, evolution, eye, hand, image analysis, lead, medicine, microscope, phase contrast micro… Meer...

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ISBN: 9789401014144

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EAN (ISBN-13): 9789401014144
Verschijningsjaar: 12
Uitgever: Springer

Boek bevindt zich in het datenbestand sinds 2015-06-10T13:56:56+02:00 (Amsterdam)
Detailpagina laatst gewijzigd op 2020-02-26T14:33:25+01:00 (Amsterdam)
ISBN/EAN: 9789401014144

ISBN - alternatieve schrijfwijzen:
978-94-010-1414-4
alternatieve schrijfwijzen en verwante zoekwoorden:
Titel van het boek: light microscopic techniques biology and medicine


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Auteur: J. James
Titel: Light microscopic techniques in biology and medicine
Uitgeverij: Springer; Springer Netherland
336 Bladzijden
Verschijningsjaar: 2012-12-06
Dordrecht; NL
Taal: Engels
149,79 € (DE)
154,00 € (AT)
177,00 CHF (CH)
Available
XII, 336 p.

EA; E107; eBook; Nonbooks, PBS / Medizin/Nichtklinische Fächer; Anatomie; Verstehen; Laboratory; biology; care; evolution; eye; hand; image analysis; lead; medicine; microscope; phase contrast microscopy; production; radiation; radiography; treatment; C; Anatomy; Veterinary Medicine/Veterinary Science; Anatomy; Veterinary Science; Medicine; Tiermedizin; BB

I: Conventional techniques of microscopic observation.- 1 Some Essentials of Geometrical Optics.- Geometrical optics and wave optics.- Resolving power and the eye.- The simple microscope.- Lens aberrations.- Properties of lens combinations.- The compound microscope.- Suggestions for further reading.- 2 From Optical Principle to a Practical Instrument.- Some notes about the history of the microscope.- The stand and its parts.- Stereoscopic vision and the microscope.- Literature cited and suggestions for further reading.- 3 Objectives.- Numerical aperture.- Immersion-objectives.- The cover glass effect.- Types of objective.- Qualifications and performance of an objective.- Special objectives.- — mirror- or reflecting objectives.- — objectives for use with invisible light.- — photomicrographic objectives.- Literature cited and suggestions for further reading.- 4 Eyepieces or Oculars.- Main types of eyepiece.- Exit pupil and the eye.- Eyepiece and field of view.- The meaning of eyepiece magnification.- Special types of eyepiece.- Literature cited and suggestions for further reading.- 5 Relation of Objective and Eyepiece.- Resolving power.- Objective, eyepiece and the eye.- Depth of field.- Literature cited and suggestions for further reading.- 6 Condenser and Illumination.- The function of the condenser.- Critical illumination and Köhler illumination.- Types of condenser.- Special types of illumination; incident illumination.- The light source.- Literature cited and suggestions for further reading.- 7 Specimen, Microscope and Observer; Microscopy in Practice.- The microscopic object as an optical model.- The technique of microscopic observation.- — Position of the observer and the placing of the microscope.- — General rules for setting up a microscope.- — Use of immersion objectives.- — Light and illumination in practice.- — On the way through the object.- — Microscopy for observers wearing spectacles.- Maintenance and small technical difficulties.- — Care of the stand and the cleaning of optical components.- — Frequently occurring minor technical troubles.- Literature cited and suggestions for further reading.- II: Advanced techniques of microscopy.- 8 Special Techniques of Illumination.- Oblique illumination.- Dark-field illumination.- Fluorescence microscopy.- — General principles.- — Optical arrangements with a fluorescence microscope.- Literature cited and suggestions for further reading.- 9 Special Techniques of Image Formation.- Phase contrast microscopy.- — Basic principles.- — Practical realization of the phase contrast principle.- — Some further details about the phase contrast image with different objects.- Interference microscopy.- — Basic principles; the meaning of a refractive index.- — Interferometric measuring systems.- — Differential interference contrast.- Polarization microscopy.- — Basic principles of birefringence.- — The polarization microscope.- — Some applications of polarization microscopy in biological research.- Literature cited and suggestions for further reading.- 10 Recording and Reproduction of Microscopic Images.- Photomicrography.- — General principles.- — Photomicrographic equipment.- — The photomicrographic exposure.- — Contrasts in the negative.- — Colour photomicrography.- Some special techniques in photomicrography.- — Microflash.- — Stereophotomicrography.- — Holography.- — Cinemicrography.- Other techniques for registration and reproduction of microscopic images.- — Drawing devices.- — Microprojection.- — Television-microscopy.- Literature cited and suggestions for further reading.- 11 Measurements with the Microscope.- General introduction.- Morphometric analysis.- — Measurement of length in a focussing plane.- — Measurement of distances along the optical axis.- — Measurement of length oblique to the optical axis.- Measurement of areas and volumes.- — Measurement of areas.- — Measurement of volumes; stereology.- Automatic and semi-automatic image analysis.- Microspectrophotometry and microphotometry.- — Microspectrophotometry.- — Microphotometry.- Microspectrofluorometry and microfluorometry.- Literature cited and suggestions for further reading.- 12 Microscopy with Invisible Electromagnetic Radiation.- Microscopy and the electromagnetic spectrum.- Ultraviolet microscopy.- Infrared microscopy.- Use of X-rays.- — Some fundamental properties of X-rays and their applications.- — X-ray microscopy.- — Contact microradiography.- — Projection-microradiography.- Literature cited and suggestions for further reading.- Appendix I Table of refractive indices.- Appendix II Four-lingual vocabulary of some commonly used technical terms in microscopy.

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